Эпигаллокатехин egcg лечебные эффекты Кожные болезни Сахарный диабет Ожирение Научные исследования Укрепления здоровья эффекты зеленого чая




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The therapeutic effects of EGCG on vitiligo.


Zhu Y1, Wang S1, Lin F1, Li Q2, Xu A3.

Author information


  • 1Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou 310009, PR. China.

  • 2Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, PR China.

  • 3Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou 310009, PR. China. Electronic address: xuaiehz@msn.com.

Abstract


Epigallocatechin-3-gallate (EGCG) is one of the main chemical constituents of green tea, which has been used as an important traditional Chinese medicine. Green tea has anti-inflammatory, anti-oxidant, and immunomodulatory properties. However, the effects of EGCG on vitiligo are not known. We assessed the role of EGCG in vitiligo induced by monobenzone in mice. We demonstrated that EGCG: delayed the time of depigmentation; reduced the prevalence of depigmentation; and decreased the area of depigmentation. Examination of depigmented skin treated with EGCG by reflectance confocal microscopy suggested increased numbers of epidermal melanocytes and histologic examination showed decreased perilesional accumulation of CD8(+) T cells. To further investigate the mechanism of the anti-inflammatory effects of EGCG, levels of inflammatory mediator tumor necrosis factor (TNF)-α, interferon (IFN)-γ and interleukin (IL)-6 were tested by enzyme-linked immunosorbent assay. Serum cytokine levels were significantly decreased after administration of EGCG compared with the model group. These results suggested that EGCG may have protective effects against vitiligo, and that it could contribute to suppression of activation of CD8(+) T cells and inflammatory mediators. Based on these results, 5% EGCG was considered to be the most suitable concentration for treating vitiligo, and was used for further study. In addition, we investigated the gene-expression profile of this model in relation to EGCG. Using a 4×44K whole genome oligo microarray assay, 1264 down-regulated genes and 1332 up-regulated genes were recorded in the 5% EGCG group compared with the model group, and selected genes were validated by real-time polymerase chain reaction. Our study demonstrated that EGCG administration was significantly associated with a decreased risk of vitiligo. EGCG could be a new preventive agent against vitiligo in the clinical setting.

Digallate димеров (-)-эпигаллокатехин галлат инактивировать herpes simplex virus.

Топические бактерицидные средства являются потенциально альтернативный метод вакцины для сокращения распространения herpes simplex virus (HSV). Ранее мы показали (S. Liu et al., Биохим. Biophys. Acta 1723:270-281, 2005), что катехин (-)-эпигаллокатехин галлат (EGCG) инактивирует ВПГ при нейтральном рН, однако, функционировать в женских половых путей EGCG также должны быть эффективными при кислых значениях рН. EGCG инактивированной ВПГ-1 и ВПГ-2 при рН 8,0 на 3 log(10) 4 log(10), но оказался неэффективным при рН 5.7. В EGCG digallate димеров theasinensin A, P2 и theaflavin-3,3'-digallate (TF-3) инактивированной обоих вирусов на 3 log(10) 4 log(10) при рН 5,7 и 5 log(10) при рН 8.0. TF-3 инактивированных HSV-1 и HSV-2, 4-5 log(10) в диапазоне рН 4,0-5,7. Димеры с одной галлат части было противовирусной активности промежуточных между деятельностью EGCG и digallate димеров. Конфокальная и электронная микроскопия показали, что theasinensin а не повреждение клеток Vero. Все EGCG димеров инактивированной оболочечных вирусов с класса I, класса II и класса III (HSV-1, HSV-2) синтеза белков более эффективно, чем мономерный EGCG. EGCG было никакой активности против nonenveloped вирусы проверял, но TF-3 снижение титра в 4 из 5 nonenveloped вирусов ≅2 до 3,5 log(10). Результаты также показали, что ВПГ-1 гликопротеина в (gB) была агрегирована более быстро theasinensin чем EGCG, которые, взятые вместе с nonenveloped virus данных, свидетельствует о том, что димеры могут ингибировать функции вирусных белков, необходимых для инфекции. Digallate димеров EGCG, кажется, есть отличный потенциал, как микробицидности агентов против ВПГ в кислых и нейтральных pHs.



Antimicrob Agents Chemother. 2011 Dec;55(12):5646-53. doi: 10.1128/AAC.05531-11. Epub 2011 Sep 26.

Digallate dimers of (-)-epigallocatechin gallate inactivate herpes simplex virus.


Isaacs CE1, Xu W, Merz G, Hillier S, Rohan L, Wen GY.

Author information


  • 1Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314, USA. chisi@rcn.com

Abstract


Topical microbicides are potentially an alternative method to vaccines for reducing the spread of herpes simplex virus (HSV). We have previously shown (S. Liu et al., Biochim. Biophys. Acta 1723:270-281, 2005) that the catechin (-)-epigallocatechin gallate (EGCG) inactivates HSV at neutral pH; however, to function in the female genital tract EGCG must also be effective at acidic pH. EGCG inactivated HSV-1 and HSV-2 at pH 8.0 by 3 log(10) to 4 log(10) but was ineffective at pH 5.7. The EGCG digallate dimers theasinensin A, P2, and theaflavin-3,3'-digallate (TF-3) inactivated both viruses by 3 log(10) to 4 log(10) at pH 5.7 and as much as 5 log(10) at pH 8.0. TF-3 inactivated HSV-1 and HSV-2 by 4 to 5 log(10) in the pH range of 4.0 to 5.7. Dimers with one gallate moiety had antiviral activity intermediate between the activities of EGCG and digallate dimers. Confocal and electron microscopy showed that theasinensin A did not damage Vero cells. All EGCG dimers inactivated enveloped viruses with class I, class II, and class III (HSV-1, HSV-2) fusion proteins more effectively than did monomeric EGCG. EGCG had no activity against the nonenveloped viruses tested, but TF-3 reduced the titer of 4 of 5 nonenveloped viruses by ≅2 to 3.5 log(10). Results also showed that HSV-1 glycoprotein B (gB) was aggregated more rapidly by theasinensin A than EGCG, which, when taken together with the nonenveloped virus data, suggests that dimers may inhibit the function of viral proteins required for infectivity. Digallate dimers of EGCG appear to have excellent potential as microbicidal agents against HSV at acidic and neutral pHs.
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